UCSF GI DIVISION FACULTY

Young S. Kim, M.D.

Professor of Medicine and Pathology
Veterans Affairs Medical Center

Address:

VAMC 151-M2
Veteran Affairs Medical Center
San Francisco, CA 94121

Email:

Active Projects:

1. Study of cell-lineage associated transcription factors, HATH1 and SOX2 in the regulation of mucin genes in colonic neoplasia
2. Analysis of genetic and epigenetic changes in serrated pathways of colorectal carcinogenesis

Techniques in Use:

1. Transfection studies using HATH1 and SOX2 expression vectors and MUC2 and MUC5AC mucin gene promoters of varying length. Electrophoretic mobility shift assays and CHIP assays. RT-PCR and Western blots. Upregulation of HATH1 and SOX2 levels in cell lines using adenoviral expression vectors. Immunohistochemistry and in situ hybridization.
2. Microdissection of colorectal cancer and polyp tissue sections and cultured cancer cell lines. DNA, RNA extraction, RT-PCR, and LOH analysis. Analysis of CpG island methylator phenotypes and microsatellite instability status. Mutation analysis of KRAS and BRAF.

Recent Publications:

Tanaka H, Deng G, Matsuzaki K, Kakar S, Kim G, Miura S, Sleisenger MH, Kim YS. BRAF mutation, CpG island methylator phenotype and microsatellite instability occur more frequently and concordantly in mucinous and non-mucinous colorectal cancer. Int. J. Cancer, 118:2765-2771, 2006.

Deng G, Nguyen A, Tanaka H, Matsuzaki K, Bell I, Mehta KR, Terdiman JP, Waldman FM, Kakar S, Gum J, Crawley S, Sleisenger MH, Kim YS. Regional hypermethylation and global hypomethylation are associated with altered chromatin conformation and histone acetylation in colorectal cancer. Int. J. Cancer, 118:2999-3005, 2006